Isolation of developmentally regulated genes by differential display screening of cDNA libraries
نویسندگان
چکیده
منابع مشابه
Isolation of developmentally regulated genes by differential display screening of cDNA libraries.
mRNA differential display RT-PCR has been extensively used for the isolation of genes differentially expressed between RNA populations. We have assessed its utility for the identification of developmentally regulated genes in plasmid cDNA libraries derived from individual tissues dissected from early mouse embryos. Using plasmid Southern blot hybridisation as a secondary screen, we are able to ...
متن کاملIsolation and characterization of recombinant cDNA clones corresponding to developmentally regulated genes in pig liver.
To identify clones that corresponded to developmentally regulated genes in pig liver, recombinant cDNA libraries were constructed from early fetal (d 40), late fetal (d 110) and adult liver mRNA. Each library was screened by probing with total cDNA prepared from mRNA at different stages of development. Nine clones that gave distinct developmental patterns when hybridized to liver RNA isolated f...
متن کاملDesign and screening of M13 phage display cDNA libraries.
The last decade has seen a steady increase in screening of cDNA expression product libraries displayed on the surface of filamentous bacteriophage. At the same time, the range of applications extended from the identification of novel allergens over disease markers to protein-protein interaction studies. However, the generation and selection of cDNA phage display libraries is subjected to intrin...
متن کاملIdentification of Thymus Specific and Developmentally Regulated Genes by an Improved Version of the mRNA Differential Display Technique
During embryogenesis in mouse, the thymus is seeded by waves of hematopoietic stem cells that provide the first peripheral T lymphocytes after birth. It is known that embryo thymocytes and adult thymocytes have different phenotypic and functional features. The identification of genes expressed in the thymus only during embryogenesis would help to understand the molecular basis underlying these ...
متن کاملDifferential methylation during maize leaf growth targets developmentally regulated genes.
DNA methylation is an important and widespread epigenetic modification in plant genomes, mediated by DNA methyltransferases (DMTs). DNA methylation is known to play a role in genome protection, regulation of gene expression, and splicing and was previously associated with major developmental reprogramming in plants, such as vernalization and transition to flowering. Here, we show that DNA methy...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1998
ISSN: 1362-4962
DOI: 10.1093/nar/26.19.4538